TASK 7 MIND MAP OF VITAMINS

TASK 6 MACHAELIS MENTEN & LINEWEAVER BURK PLOT

For Michaelis-Menten plot:

This data is obtained from the conversion of cellulose to cellobiose by endoglucanase enzyme:

Concentration of Substrate [S]	0.5	0.75	2	4	6	8	10
Velocity (V)	0.075	0.09	0.152	0.196	0.21	0.214	0.23

a) Using the above data, plot a graph on a properly labelled and titled Michaelis-Menten plot.

b) From the graph, calculate the Vmax and Km value of the enzyme.

For Lineweaver Burke plot:

This data is obtained from the conversion of amylopectin to β-limit dextrin by isoamylase enzyme:

[S] (mM)	2.5	1	0.714	0.526	0.250
Velocity (mM.s-1)	0.588	0.500	0.417	0.370	0.256

a) Using the above data, recalculate and plot a graph on a properly lablled & titled Lineweaver Burk plot.

b) Using the graph, calculate the Vmax and Km value of the enzyme.

c) On the grapgh above, draw and label the shift in the Lineweaver Burk plot that would occur if an uncompetitive inhibitor was added if an uncompetitive inhibitor was added to your enzyme reaction

TASK 5 FIBROUS VS GLOBULAR

TASK 3 INFOGRAPHIC NON COVALENT INTERACTIONS

TASK 4: SOLVING HENDELSON-HASSELBACH EQUATION

Calculate the pH of a mixture of 0.1 M acetic acid (CH3COOH) and 0.2 M sodium acetate (CH3COO-). (The pKa of acetic is 4.76).

CH3COOH   ⇌ CH3COO– + H+

TASK 1 SELF REFLECTION INTRODUCTION OF BIOCHEMISTRY

Hello, I am Nurjannah Dalilah a student from G6 class which taught by Dr Dayang Salwani. The first class with Dr Dayang was so much fun as I was able to gain new knowledge and interesting perspectives in biochemistry. Firstly, I can define what biochemistry is. Biochemistry is the discipline that uses the principles and language of chemistry to explain biology.  Moreover, biochemistry describe the structure, organization, and function of living organisms in molecular terms. In order to understand the life on the molecular level, we must first have a knowledge of the chemical structures of biological molecules, biological function of the molecules and understanding of bioenergetics – study of energy flow in cells.

         Furthermore, biochemistry can be divided into three areas of study. First, conformational which investigate structure and three-dimensional arrangements of biomolecules, for instance macromolecules and proteins structure. Second, metabolism that convey about biochemical pathways like Kreb Cycle, energy production and utilization such as glycolysis. Third is informational which scrutinize about the language for communication inside and between cells, for example signal transduction.

          Besides that, biochemistry also play a vital role and importance in medicine and clinical. With the technical of this biochemistry, we able to diagnostic and monitoring of diseases and discover the development of new and improved antibiotics and medications such as chemotherapy agents and designer drugs. While in the agriculture field, with the biochemistry knowledge, we able to establish herbicides and pesticides production and can be used in transgenic crops. Whereas in industry, with the biochemistry research discipline which applicable to form the production of industrial and research materials like enzyme.

          I like the way Dr Dayang Salwani teach us in the class. The lecture was contented and I able to understand easily with information stated in the lecture slide. I look forward to learning more about biochemistry because to me this is important and the scope is so rich that it brings many benefits to our daily life.